昆山做企业网站,免费设计素材下载,长沙市网站制作,济宁网站建设 优化培训EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher推出的CUTRUN级别的Histone H3K4me2 Antibody符合EpiCypher的批次特异性SNAP-CertifiedTM标准#xff0c;在CUTRUN中具有特异性和高效的靶点富集。通过SNAP-CUTANA™K-Me…EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher推出的CUTRUN级别的Histone H3K4me2 Antibody符合EpiCypher的批次特异性SNAP-CertifiedTM标准在CUTRUN中具有特异性和高效的靶点富集。通过SNAP-CUTANA™K-MetStat Panel的spike-in对照确定(EpiCypher 19-1002Fig. 1)与相关组蛋白PTM的交叉反应性20%在500k和50k起始细胞中一致的基因组富集证实了高靶向效率(Fig. 2-4)。该抗体靶向的H3K4me2在转录活性基因的启动子和细胞发育过程中表达的基因中富集[1]。 [1] Pekowkska et al. Genome Res. (2010). PMID: 20841431. 产品详情
反应种属: Human, Mouse, Wide Range (Predicted)
宿主来源: Rabbit
实验应用: CUTRUN, ICC, WB
免疫原: A synthetic peptide corresponding to histone H3 dimethylated at lysine 4
克隆性: Monoclonal
表达系统: HEK293 cells
保存温度: Stable for 1 year at -20°C from date of receipt
运输温度: Frozen cold packs.
产品形式: Protein A affinity-purified antibody in PBS, 0.09% sodium azide, 1% BSA, and 50% glycerol
如需购买EpiCypher公司产品或咨询产品计算问题请联系EpiCypher代理商欣博盛生物。 验证数据 Figure 1: SNAP specificity analysis in CUTRUN
CUTRUN was performed as described above. CUTRUN sequencing reads were aligned to the unique DNA barcodes corresponding to each nucleosome in the K-MetStat panel (x-axis). Data are expressed as a percent relative to on-target recovery (H3K4me2 set to 100%). Figure 2: CUTRUN genome wide enrichment
CUTRUN was performed as described above. Sequence reads were aligned to 18,793 annotated transcription start sites (TSSs ± 2 kbp). Signal enrichment was sorted from highest to lowest (top to bottom) relative to the H3K4me2 - 50k cells sample (all gene rows aligned). High, medium, and low intensity are shown in red, yellow, and blue, respectively. H3K4me2 antibody produced the expected TSS enrichment pattern, which was consistent between 500k and 50k cells and greater than the IgG negative control. Figure 3: H3K4me2 CUTRUN representative browser tracks
CUTRUN was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Similar results in peak structure and location were observed for both cell inputs. Figure 4: Antibody efficiency analysis in CUTRUN using cell input correlation
CUTRUN was performed as described above. Genome-wide correlation analysis was performed to compare H3K4me2 antibody enrichment using 500k cell and 50k cell inputs. The log of the number of reads per 75 bp binned region across the genome is plotted for both samples. CUTRUN data generated using this H3K4me2 antibody are highly correlated between the two cell inputs (Pearson correlation r 0.942), indicating high efficiency of H3K4me2 antibody target recovery. Figure 5: Immunocytochemistry
ICC of HeLa cells using 2 µg/mL of H3K4me2 antibody (red). Actin filaments were labeled with fluorescein phalloidin (green). Figure 6: Western blot data
Recombinant histone H3.3 (Lane 1) and acid extracts of HeLa cells (Lane 2) were blotted onto PVDF and probed with 0.025 µg/mL of H3K4me2 antibody. 订购详情 货号 产品名称 规格 13-0027 Histone H3K4me2 Antibody, SNAP-Certified™ for CUTRUN / 欣博盛生物 100 µg
